Topical T3 thyroid hormone greatly accelerates chronic wound healing (diabetic foot ulcer)

cs3000

cs3000

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Chronic wounds, human trial, massive beneficial effect
https://pubmed.ncbi.nlm.nih.gov/37226792/

16 days for complete wound closure with topical t3 applied vs 60 days for the honey cream treated group
*The mean time to complete wound closure in the routine care group was about 60.6 days, while it was 15.9 and 16.4 days in T3 and T3/Ins groups, respectively

At the time of study termination, all participants in each of the T3 or T3/Ins groups were free of symptoms based on the REEDA score**, while about 40% of participants in the control group were detected with each of grades 1, 2, or 3.
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https://academic.oup.com/endo/article/145/5/2357/2878224?login=false
. To examine TH requirement for optimal wound healing, we contrasted TH-deficient vs. ip T3-treated mice.
Four days after wounding, ip T3-treated mice had twice the degree of wound closure as hypothyroid mice (P < 0.001).
By RT-PCR, K6a and K17 gene expression from control mouse skin was greater than from hypothyroid mouse skin: 5- (P < 0.001) and 1.7-fold (P < 0.05), respectively.

T3 is necessary for the keratinocyte proliferation required for optimal wound healing. T3 exerts influence by stimulating expression of the wound-healing keratin genes. Thus, for hypothyroid patients undergoing surgery that cannot be delayed until euthyroidism is achieved, our data support T3 treatment for the perioperative period.
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1710426900812.png<- systemic effect delivered i.p so not just when used on skin directly

^ used on skin in mice, dose 150ng
Daily topical application of 150 ng T3 resulted in 58% greater wound closure relative to wounds on the same animals receiving vehicle alone
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& this one is interesting - showing thyroxine (T4) also works to restore wound healing and when conditions are set up for hypoxic and high oxidative stress tissue which otherwise prevented healing
link.springer.com

Thyroxine restores severely impaired cutaneous re-epithelialisation and angiogenesis in a novel preclinical assay for studying human skin wound healing under “pathological” conditions ex vivo - Archives of Dermatological Research

Impaired cutaneous wound healing remains a major healthcare challenge. The enormity of this challenge is compounded by the lack of preclinical human skin wound healing models that recapitulate selected key factors underlying impaired healing, namely hypoxia/poor tissue perfusion, oxidative...
link.springer.com link.springer.com

we sought to further mimic “pathological” wound healing conditions by culturing experimentally wounded, healthy full-thickness frontotemporal skin from three healthy female subjects for three days in either serum-free supplemented Williams’ E medium or in unsupplemented medium under “pathological” conditions (i.e. hypoxia [5% O2], oxidative damage [10 mM H2O2], absence of insulin, excess glucose)

Indeed, thyroxine administration sufficed to rescue re-epithelialisation (p < 0.001) and promoted both epidermal keratinocyte proliferation (p < 0.01) and angiogenesis in terms of CD31 immunoreactivity and CD31 positive cells under “pathological” conditions (p < 0.001) ex vivo.
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(and also boosted in standard tissue environment)
1710427917358.png
 
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cs3000 said:
Chronic wounds, human trial, massive beneficial effect
https://pubmed.ncbi.nlm.nih.gov/37226792/

16 days for complete wound closure with topical t3 applied vs 60 days for the honey cream treated group

https://academic.oup.com/endo/article/145/5/2357/2878224?login=false

View attachment 3<- systemic effect delivered i.p so not just when used on skin directly

^ used on skin in mice, dose 150ng



& this one is interesting - showing thyroxine (T4) also works to restore wound healing and when conditions are set up for hypoxic and high oxidative stress tissue which otherwise prevented healing

(and also boosted in standard tissue environment)
View attachment 5
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Do you have any idea what might be an appropriate topical dose for a diabetic foot ulcer in a human?
 
latte said:
Do you have any idea what might be an appropriate topical dose for a diabetic foot ulcer in a human?
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id think a few mcg would work by the looks of it once you hit a threshold dose more has the same effect (but going too high lowers t4 more) ,
but would have to disperse it in something that allows it to spread it around wound , especially if product uses ethanol not great putting undiluted ethanol on damaged skin,
the t3 is soluble in warm water , up to 4mg dissolves in 1 litre at 37 degrees apparently and only micrograms are used
or if the product uses fatty acids maybe olive oil could work to distribute which apparently has its own wound healing properties topically
Mice treated with 3.8 mcg of topical T3 daily did not have wound healing acceleration beyond that of the mice treated with 150 ng T3 daily
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Something interesting, t3 on skin only gets slightly into the system. but here it lowered t4 levels (so the local t3 signalled lower t4 production broadly, where system t3 levels stayed stable with a little increase at highest dose)
(i wonder if the lower t4 effects signalling of t3 positively)
Serum T4 levels were measured in all mouse groups to ascertain the impact of T3 treatment on the thyroid axis. Lower T4 levels were interpreted to mean that there was systemic absorption of T3 suppressing the endogenous axis. Control T4 levels were 4.2 0.4 g/dl. T4 levels in mice treated with 150 ng T3 topically were 3.0 0.9 (P 0.3 vs. control), T4 levels in 3.8 mcg-treated mice were 1.8 0.2 (P 0.04), T4 levels in the ip T3-treated mice were 1.6 0.4 (P 0.01).

Mice treated with topical T3 did not have significant deviations from baseline with regard to their T3 levels. The highest recorded T3 level in the topically treated mice was in those mice treated with 3.8 mcg of T3. One hour after treatment, the 3.8 mcg T3 treatment mice had peak serum T3 levels of 85 +-15 ng/dl (P 0.6 relative to control T3 levels which were 70 +-10 ng/dl)
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i just put up a post of some other relevant stuff for skin healing
 
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